FIGURE 4.

Dissociation of the ARHGAP17-CIP4 complex inhibits cell migration. HeLa cells were seeded onto culture inserts for wound healing assays. After transfection of ARHGAP17-EGFP (wt), ARHGAP17-S702A-EGFP (SA), or ARHGAP17-S702E-EGFP (SE) the inserts were removed resulting in a cell-free gap between cells. The size of this open area was measured by microscopy, and data were normalized relative to the size of the starting area. Shown are means +S.E. of five independent experiments. The asterisk indicates statistical significance between SA and SE mutants of ARHGAP17 (p < 0.05, ANOVA with Bonferroni correction).