TABLE 5.

Binding kinetics of anti-B7-H3 IgG constructs

Error values are shown as S.E., and R_max represents the maximum response units (RU) measured for the highest concentration of each sample.

Antibodies	4Ig-B7-H3					2Ig-B7-H3
	ka	kd	KD	Rmax	χ2	ka	kd	KD	Rmax	χ2
	s−1m−1	s−1	nm	RU	RU2	s−1m−1	s−1	nm	RU	RU2
m8H9	1.846 ± 0.006 × 104	1.650 ± 0.002 × 10−4	8.9	87.34	0.153	2.776 ± 0.011 × 104	2.244 ± 0.019 × 10−5	0.81	76.5	0.150
ch8H9	2.834 ± 0.030 × 104	2.180 ± 0.010 × 10−4	7.7	66.49	2.69	3.210 ± 0.025 × 104	8.729 ± 0.046 × 10−5	2.7	68.51	0.724
ch8H9-6m	3.349 ± 0.014 × 104	1.229 ± 0.002 × 10−4	3.7	107.1	0.550	6.890 ± 0.018 × 104	4.254 ± 0.026 × 10−5	0.62	96.84	0.844
hu8H9	2.046 ± 0.052 × 104	6.770 ± 0.023 × 10−4	33	41.55	0.472	1.347 ± 0.020 × 104	6.069 ± 0.009 × 10−4	45	57.89	0.117
hu8H9-6m	2.342 ± 0.020 × 104	2.979 ± 0.005 × 10−4	13	82.60	0.572	3.394 ± 0.011 × 104	1.711 ± 0.002 × 10−4	5.0	80.61	0.211