FIGURE 3.
kpol, Kd, and kpol/Kd comparison of the four RT enzymes at three different sites on the 40-mer RNA template. A–C, the maximum incorporation rates (kpol) (A), the dNTP binding affinity (Kd) (B), and the incorporation efficiency (kpol/Kd) (C) of RT protein from Vpx non-coding (black bars) and Vpx coding (open bars) lentiviruses at the three different sites described in the legend for Fig. 1 were determined 3–7 times, and the average values are shown with error bars representing S.E. -Fold changes are indicated by brackets above the bars, and statistical significance from an unpaired Student's t test is indicated as: NS, not significant; *, p < 0.05; **, p < 0.01; ***, p < 0.001.