The expression of p16INK4A, SALNR, miR-181a, and miR-22 and location of NF90 in colon adenomas. qRT-PCR analysis of p16INK4A (A), SALNR (B), miR-181a (C), and miR-22 (D) expression in colon adenomas and adjacent normal colon tissues. Levels are normalized to GAPDH (p16INK4A and SALNR) or U6 (miR-181a and miR-22) expression and represented as means ± S.D. (error bars). E, immunofluorescence localization of NF90 in colon adenomas. Fibrillarin is the nucleolar marker, and DAPI staining was used to indicate the cell nucleus.