FIGURE 6.

SALNR interacts with NF90. A, subcellular location of SALNR. The antisense of SALNR was used for a control. Fibrillarin is the nucleolar marker, and DAPI staining was used to indicate the cell nucleus. B, identification of SALNR-associated target proteins in young 2BS cells by RNA pull-down assay. The bands specific to sense SALNR were detected with mass spectrometry. C, Western blot analysis of the interaction of SALNR with NF90. Proteins pulled down were detected by NF90 antibody. D, RIP analysis of NF90 binding to SALNR in vivo. RNA was immunoprecipitated using anti-NF90 antibody in young 2BS cells, with IgG as a negative control, and analyzed by qRT-PCR. Error bars, S.D.