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. 2015 Oct 7;290(50):30006–30017. doi: 10.1074/jbc.M115.677328

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FIGURE 6. — Get3 binds to one-half of the Get4/5 heterotetramer. A, SEC-multiangle light scattering (MALS) analysis of the Get3·Get4/5 complex (ScGet/3/4/5). B, model depicting the N-ethylmaleimide (NEM) accessibility of a solvent-exposed Cys residue (S48C) on Get4/5 alone and in complex with Get3. C, results of the alkylation assay shown in B. D, ATP-bound structure of Get3·Get4/5N (Protein Data Bank code 4PWX) demonstrating the location of Q34C within the Get3·Get4/5 interface. E, results of the PEGylation assay with Get4 Q34C/5 alone or in complex with Get3. RIU, refractive index units. Sc, S. cerevisiae.