FIGURE 1.

Microarray analysis to identify hypoxia-regulated genes in primary myoblasts. A, genes affected by hypoxia (-fold change ≥ 2 and p ≤ 0.01) in two biological replicates were merged and filtered, including 641 up-regulated genes and 224 down-regulated genes. B, heat map showing selective pathways and genes regulated by hypoxia. Green indicates down-regulated genes and pathways, and red (orange) shows up-regulated genes and pathways. C, -fold increases of Bhlhe40 under hypoxia compared with normoxia. D, relative protein levels of Bhlhe40 under normoxia and hypoxia.