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. 2015 Oct 23;290(50):29993–30005. doi: 10.1074/jbc.M115.670018

FIGURE 6.

FIGURE 6.

Determination of role of LdRab1 in HbR trafficking Leishmania. A, to determine the role of LdRab1 in the trafficking of HbR, Leishmania promastigotes overexpressing GFP-LdRab1:WT, GFP-LdRab1:S22N, or GFP-LdRab1:Q67L were transfected with HbR-RFP as described under “Experimental Procedures.” Cells were examined by confocal microscopy. Green, GFP-LdRab1; red, HbR-RFP. Results are representative of three independent experiments. B, to determine the endogenous HbR present in the flagellar pocket in respective Leishmania, GFP-LdRab1:WT-, GFP-LdRab1:S22N-, or GFP-LdRab1:Q67L-overexpressing cells were stained with anti-HBR-N antibody as described under “Experimental Procedures.” Cells were examined by confocal microscopy. Green, GFP-LdRab1; red, HbR. Results are representative of three independent experiments. C, quantitative analysis of mean fluorescence intensity of HbR in the indicated cell types. Results are expressed as mean ± S.D. (error bars).