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. 2015 Oct 23;290(50):29993–30005. doi: 10.1074/jbc.M115.670018

FIGURE 7.

FIGURE 7.

Determination of role of LdRab1 in conventional secretory pathway in Leishmania. A, to determine the role of LdRab1 in the secretion of SAP by Leishmania, the cell association or release of SAP in the spent media by L. donovani promastigotes overexpressing LdRab1:WT or its mutant proteins was determined as described under “Experimental Procedures.” The amount of p-nitrophenolate ion released or cell-associated by respective Leishmania was calculated, and results were expressed as μm p-nitrophenol ± S.D. (error bars) by respective Leishmania from three independent experiments. B, to determine the role of LdRab1 in the trafficking of gp63, Leishmania promastigotes overexpressing GFP-LdRab1:WT, GFP-LdRab1:S22N, or GFP-LdRab1:Q67L were transfected with RFP-Ldgp63 as described under “Experimental Procedures.” Cells were examined by confocal microscopy. Green, GFP-LdRab1; red, RFP-Ldgp63. Results are representative of three independent experiments. C, to determine the role of LdRab1 in the secretion of endogenous gp63 and HbR by Leishmania, the levels of gp63 or HbR associated with cells and secreted in the spent medium by respective L. donovani promastigotes were determined by Western blot analysis using specific antibodies as described under “Experimental Procedures.” Results are representative of three independent experiments.