FIG. 2.

A. phagocytophilum-infected neutrophils (polymorphonuclear leukocytes [PMN]) exhibit reduced ROS production. Host cell-free A. phagocytophilum (Ap) cells were added to 100,000 neutrophils at approximate ratios of 10 and 30 A. phagocytophilum cells/neutrophil and incubated for 60, 120, or 240 min. Neutrophils incubated with HL-60 lysate served as a control (ctrl). At various numbers of minutes p.i. (mpi), PMA (A) or FMLP (B) was added, and ROS production was indirectly measured as the change in DCL over time. The data are presented in RLU. The results are representative of at least 10 independent experiments.