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Neither gp91phox nor p22phox colocalizes to the A. phagocytophilum (Ap) vacuolar membrane. Neutrophils were examined by immunoelectron microscopy 240 min post-A. phagocytophilum infection as described in Materials and Methods using MAbs directed against gp91phox (A) and p22phox (B). The arrowheads highlight representative gold particles. Bar, 500 nm. The results are representative of several micrographs.
