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. 2015 Nov 24;25(2):254–265. doi: 10.1093/hmg/ddv468

Figure 3.

Figure 3.

Isoform switching of structural sarcomeric gene, TTN, owing to alternative splicing defect in RBM20 familial DCM hiPSC-CMs. (A) qRT-PCR showing endogenous expression of RBM20 in control and RBM20 hiPSC-CMs at Day 20. (B) qRT-PCR expression of TTN N2B isoform (exon 50–220). (C) qRT-PCR expression of TTN N2BA isoform (exon 107–108). Two distinct clones were examined for control and RBM20_1 hiPSC lines, represented as distinct bars. Values were normalized to the housekeeping gene GAPDH and expressed as mean ± SEM. Expression values are relative to control hiPSC-CMs (n = 3 biological repetitions with three technical replicates for each biological repetition). (D) Schematic representation of TTN N2B adult and N2BA fetal isoform, showing the region of alternative splicing (*P < 0.05; **P < 0.01; ***P < 0.001, paired Student's t test between control and RBM20).