Figure 6.
RBM20 familial DCM hiPSC-CMs are susceptible to structural damage upon β-adrenergic stimulation with norepinephrine. (A) Representative images of sarcomeric α-actinin immunostaining of single control and RBM20 DCM hiPSCs-CMs after treatment with and without NE at Day 20. Compared with controls, NE treatment-induced sarcomeric disorganization in single RBM20 DCM hiPSCs-CMs. Scale bar: 20 μm. (B) Percentage of hiPSCs-CMs with disorganized sarcomeric α-actinin staining patterns (defined as ≥25% punctate appearance) with or without NE treatment (**P < 0.001). mm2, millimeters squared.