Table 2.
mRNA expression in THP-1 cells after a co-culture with A549 epithelial monolayers. THP-1 cells were co-cultured with a monolayer of A549 cells separated by a filter insert. After 4 h, 8 h, 12 h, and 24 h, the THP-1 cells were aspirated, mRNA extracted and the expression of mRNA evaluated by qRT-PCR. The experiment has been repeated three times with consistent results. Data were correlated with mRNA expression of unstimulated THP-1 cells and statistically evaluated with a web-based program provided by the manufacturer. Bold are the values of at least 4-fold change of mRNA expression.
| Upregulated | ||||||||||||
| Symbol | 95% CI | Fold reg. | p-Value | 95% CI | Fold reg. | p-Value | 95% CI | Fold reg. | p-Value | 95% CI | Fold reg. | p-Value |
| IL8 | (147.30, 296.04) | 221.67 | 0 | (174.85, 197.32) | 186.08 | 0 | (207.99, 245.93) | 226.96 | 0 | (27.32, 30.74) | 29.03 | 0 |
| EGR1 | (82.59, 111.12) | 96.85 | 0 | (115.54, 124.96) | 120.25 | 0 | (45.21, 65.48) | 55.34 | 0 | (7.73, 18.74) | 13.23 | 0.01 |
| TNF | (58.66, 69.62) | 64.14 | 0 | (41.25, 48.81) | 45.03 | 0 | (18.15, 22.35) | 20.25 | 0 | (22.34, 29.40) | 25.87 | 0 |
| IL1B | (29.80, 55.01) | 42.41 | 0 | (32.09, 59.42) | 45.75 | 0 | (286.84, 483.44) | 385.14 | 0 | (1.43, 3.28) | 2.35 | 0.02 |
| HMOX1 | (31.26, 39.29) | 35.28 | 0 | (26.57, 30.66) | 28.61 | 0 | (25.31, 29.56) | 27.43 | 0 | (3.32, 8.68) | 6 | 0.02 |
| CCL2 | (6.93, 11.50) | 9.21 | 0 | (9.83, 10.96) | 10.4 | 0 | (31.39, 33.10) | 32.24 | 0 | (4.05, 5.20) | 4.62 | 0 |
| JUN | (6.24, 12.00) | 9.12 | 0 | (5.80, 7.05) | 6.43 | 0 | (7.27, 13.11) | 10.19 | 0 | (2.48, 4.01) | 3.25 | 0 |
| LTA | (6.94, 8.66) | 7.8 | 0 | (7.91, 9.49) | 8.7 | 0 | (5.49, 6.90) | 6.2 | 0 | (5.44, 10.86) | 8.15 | 0.01 |
| IRAK2 | (3.50, 7.43) | 5.46 | 0.01 | (4.30, 6.67) | 5.49 | 0 | (17.13, 23.24) | 20.19 | 0 | (1.38, 2.61) | 1.99 | 0.03 |
| FOS | (4.30, 6.28) | 5.29 | 0 | (5.10, 7.15) | 6.13 | 0 | (9.26, 11.60) | 10.43 | 0 | (5.49, 8.71) | 7.1 | 0 |
| IFNB1 | (2.11, 8.17) | 5.14 | 0 | (1.81, 6.41) | 4.11 | 0 | (0.00001, 9.74) | 4.77 | 0.14 | (2.12, 8.58) | 5.35 | 0 |
| ICAM1 | (3.38, 5.53) | 4.45 | 0 | (5.01, 7.21) | 6.11 | 0 | (11.25, 18.77) | 15.01 | 0 | (0.86, 1.88) | 1.37 | 0.18 |
| IL1R1 | (3.05, 3.91) | 3.48 | 0 | (3.74, 4.41) | 4.08 | 0 | (5.51, 7.17) | 6.34 | 0 | (1.73, 2.57) | 2.15 | 0 |
| CD40 | (2.08, 3.54) | 2.81 | 0 | (4.63, 7.59) | 6.11 | 0 | (3.54, 7.86) | 5.7 | 0.01 | (1.65, 2.45) | 2.05 | 0 |
| BCL3 | (2.42, 3.07) | 2.75 | 0 | (2.24, 3.24) | 2.74 | 0 | (11.64, 14.65) | 13.15 | 0 | (0.47, 0.79) | 1.59 | 0.02 |
| CD27 | (1.79, 3.54) | 2.66 | 0 | (1.10, 3.01) | 2.06 | 0.05 | (1.21, 2.68) | 1.94 | 0.02 | (2.98, 8.06) | 5.52 | 0.01 |
| NFκB1 | (2.02, 2.52) | 2.27 | 0 | (1.63, 2.04) | 1.84 | 0 | (4.10, 5.20) | 4.65 | 0 | (0.77, 1.24) | 1.01 | 0.92 |
| IL6 | (1.42, 2.98) | 2.2 | 0.04 | (2.95, 3.61) | 3.28 | 0 | (2.79, 7.21) | 5 | 0.03 | (6.43, 10.26) | 8.34 | 0 |
| MALT1 | (1.04, 2.70) | 1.87 | 0.09 | (2.11, 2.34) | 2.23 | 0 | (9.36, 10.57) | 9.96 | 0 | (1.02, 1.54) | 1.28 | 0.1 |
| TNFRSF10B | (1.61, 1.95) | 1.78 | 0 | (2.23, 2.69) | 2.46 | 0 | (4.46, 7.40) | 5.93 | 0 | (1.07, 1.38) | 1.22 | 0.04 |
| IL10 | (1.41, 1.65) | 1.53 | 0 | (1.45, 2.53) | 1.99 | 0.02 | (3.31, 7.95) | 5.63 | 0.01 | (6.43, 10.26) | 8.34 | 0 |
| IFNA1 | (1.41, 1.65) | 1.53 | 0 | (0.94, 0.96) | 1.05 | 0 | (1.40, 1.77) | 1.58 | 0 | (6.43, 10.26) | 8.34 | 0 |
| CSF2 | (1.41, 1.65) | 1.53 | 0 | (0.39, 2.57) | 1.48 | 0.36 | (1.13, 1.41) | 1.27 | 0.02 | (6.43, 10.26) | 8.34 | 0 |
| CSF3 | (1.41, 1.65) | 1.53 | 0 | (0.94, 0.96) | 1.05 | 0 | (1.13, 1.41) | 1.27 | 0.02 | (6.43, 10.26) | 8.34 | 0 |
| FASLG | (1.41, 1.65) | 1.53 | 0 | (0.94, 0.96) | 1.05 | 0 | (1.13, 1.41) | 1.27 | 0.02 | (6.43, 10.26) | 8.34 | 0 |
| IFNG | (1.41, 1.65) | 1.53 | 0 | (0.94, 0.96) | 1.05 | 0 | (1.13, 1.41) | 1.27 | 0.02 | (6.43, 10.26) | 8.34 | 0 |
| TLR3 | (1.41, 1.65) | 1.53 | 0 | (1.07, 1.15) | 1.11 | 0 | (1.13, 1.41) | 1.27 | 0.02 | (6.43, 10.26) | 8.34 | 0 |
| IL1A | (1.19, 1.29) | 1.24 | 0 | (1.45, 2.97) | 2.21 | 0.03 | (4.49, 8.15) | 6.32 | 0.01 | (0.54, 0.77) | 1.53 | 0.01 |
| REL | (1.11, 1.19) | 1.15 | 0 | (0.93, 1.08) | 1.01 | 0.83 | (4.25, 5.27) | 4.76 | 0 | (0.52, 0.67) | 1.68 | 0 |
| Downregulated | ||||||||||||
| F2R | (0.13, 0.37) | 3.99 | 0.02 | (0.14, 0.35) | 4.14 | 0.02 | (0.09, 0.31) | 4.99 | 0.02 | (0.19, 0.63) | 2.45 | 0.06 |
| MAP3K1 | (0.25, 0.52) | 2.58 | 0 | (0.62, 0.68) | 1.54 | 0 | (0.43, 0.52) | 2.11 | 0 | (0.22, 0.27) | 4.08 | 0 |
| GJA1 | (0.40, 0.70) | 1.81 | 0.02 | (0.78, 1.34) | 1.06 | 0.75 | (3.35, 6.17) | 4.76 | 0 | (0.39, 1.01) | 1.42 | 0.21 |