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. 2004 Aug;72(8):4699–4706. doi: 10.1128/IAI.72.8.4699-4706.2004

FIG. 3.

FIG. 3.

Demonstration of A-region and P-region interaction by ELISA. One hundred nanograms of the P-region-MBP fusion polypeptide (circles) or of MBP alone (triangles) was used to coat ELISA plate wells. Twofold serial dilutions of the purified A-region-MBP fusion polypeptide starting at 1,000 ng/well were added to the coated wells, and binding of the A region to the P region or to the MBP negative control was traced with the A-region-specific MAb 3-8D.