FIG. 7.
Western blot analysis of P1 cellular location in the E. coli secB-negative mutant CK1953. Leftmost lane, P1 extracted from S. mutans NG8, used as a positive control for antibody reactivity. As indicated, lanes contain cytoplasmic (C) or periplasmic (P) extracts of E. coli MC4100 (wild type) or CK1953 (secB::Tn5) harboring pDC20 (full-length P1) or of MC4100 harboring pUC18. The membrane was reacted with a cocktail of MAbs including 5-3E, 2-8G, 3-3B, and 6-8C. Molecular weight standards, indicated on the left, correspond to Mrs of 250,000, 150,000, and 100,000.