TABLE 2.

Primers for RT-PCR

Primer RT-PCR function	Sequence	Predicted size (bp)
YopM− mutant construction
Left flanking DNA
LFup	ACATTAGAGCTCTGCCTGTCTCCGTTCTTG	1,183
LFdown	TACTATCTGCAGTGTATGGCCGCAGAGT
Right flanking DNA
RFup	TGCTGCAGACGCAAGAGCGTTCAT	1,242
RFdown	ATCTCTCGAGACGCCACCGTTGATTA
Cytokine message analysis
IFN-γ Forward	5′ TAC TGC CAC GGC ACA GTC ATT GAA 3′	406
IFN-γ Reverse	5′ GCA GCG ACT CCT TTT CCG CTT CCT 3′
TNF-α Forward	5′ ATG AGC ACA GAA AGC ATG ATC 3′	276
TNF-α Reverse	5′ TAC AGG CTT GTC ACT CGA ATT 3′
IL-1β Forward	5′ CAG GAT GAG GAC ATG AGC ACC 3′	447
IL-1β Reverse	5′ CTC TGC AGA CTC AAA CTC CAC 3′
IL-4 Forward	5′ ACG GAG ATG GAT GTG CCA AAC GTC 3′	279
IL-4 Reverse	5′ CGA GTA ATC CAT TTG CAT GAT GC 3′
IL-6 Forward	5′ GAA AAT CTG CTC TGG TCT TCT GG 3′	227
IL-6 Reverse	5′ TTT TCT GAC CAC AGT GAG GAA TG 3′
IL-10 Forward	5′ CTG CTC CAC TGC CTT GCT CTT ATT 3′	275
IL-10 Reverse	5′ GTG AAG ACT TTC TTT CAA ACA AAG 3′
IL-12 Forward	5′ GGA GAC CCT GCC CAT TGA ACT 3′	407
IL-12 Reverse	5′ CAA CGT TGC ATC CTA GCA TCG 3′
IL-15 Forward	5′ GAA TAC ATC CAT CTC GTG CTA CT 3′	422
IL-15 Reverse	5′ TTT GCA AAA ACT CTG TGA AGG 3′
IL-15Rα Forward	5′ TGC TGC TGC TGC TGT TGC TA 3′	386
IL-15Rα Reverse	5′ TGT CTC TGT GGT CAT TGC GGT AT 3′
IL-18 Forward	5′ GCC TCT AGA GTG AAC ATT ACA GAT TTA TCC CCA 3′	436
IL-18 Reverse	5′ ACC GAA TTC ACT GTA CAA CCG CAG TAA TAG GGA 3′
β-Actin Forward	5′ GTG GGC CGC TCT AGG CAC CA 3′	245
β-Actin Reverse	5′ CGC TTG GCC TTA GGG TTC AGG GGG G 3′