TABLE 3.
Average populations of CD4+ and CD8+ T cells, CD19+ B cells, GR1+ PMNs, and F4/80+ macrophages taken from infected spleen samples during infection
| Cells and infecting strain | Total no. of cells per spleen (106)a
|
|||||
|---|---|---|---|---|---|---|
| Day 1 | Day 2 | Day 3 | Day 4 | Day 5 | Day 8 | |
| CD4+ T cells | ||||||
| KIM5 | 20.4 ± 3.1 | 13.2 ± 0.7 | 10.3 ± 0.6 | 8.5 ± 3.1 | 9.3 ± 1.3 | NA |
| YopM− | 17.7 ± 3.9 | 16.4 ± 3.8 | 11.2 ± 1.0 | 12.9 ± 2.3 | 14.6 ± 2.4 | 10.5 ± 1.4 |
| Noninfected | 15.9 ± 2.5 | |||||
| CD8+ T cells | ||||||
| KIM5 | 9.4 ± 3.2 | 6.5 ± 0.3 | 4.7 ± 0.5 | 5.4 ± 0.1 | 4.3 ± 1.5 | NA |
| YopM− | 10.6 ± 1.4 | 7.8 ± 1.5 | 6.4 ± 1.0 | 9.6 ± 2.3 | 10.2 ± 1.2 | 10.8 ± 1.2 |
| Noninfected | 9.8 ± 1.2 | |||||
| CD19+ B cells | ||||||
| KIM5 | 12.4 ± 2.4 | 21.2 ± 1.0 | 22.2 ± 1.7 | 17.0 ± 4.4 | 19.1 ± 3.3 | NA |
| YopM− | 16.1 ± 4.0 | 18.5 ± 1.0 | 23.8 ± 1.2 | 19.3 ± 3.0 | 18.3 ± 4.3 | 15.9 ± 1.7 |
| Noninfected | 14.6 ± 1.8 | |||||
| GR1+ PMNs | ||||||
| KIM5 | 7.9 ± 3.8 | 9.4 ± 0.1 | 7.7 ± 0.2 | 7.4 ± 1.0 | 7.9 ± 1.0 | NA |
| YopM− | 5.4 ± 0.4 | 10.0 ± 0.4 | 11.6 ± 1.6 | 11.9 ± 3.6 | 10.2 ± 3.0 | 8.8 ± 1.0 |
| Noninfected | 7.1 ± 1.4 | |||||
| F4/80+ macrophages | ||||||
| KIM5 | 11.9 ± 4.6 | 11.6 ± 0.4 | 11.1 ± 3.0 | 15.2 ± 4.7 | 14.3 ± 1.6 | NA |
| YopM− | 14.1 ± 4.7 | 15.0 ± 7.1 | 12.3 ± 2.9 | 16.9 ± 4.4 | 19.1 ± 3.4 | 16.3 ± 4.0 |
| Noninfected | 12.6 ± 1.5 | |||||
a
Values are the number of CD45+ splenocytes expressing the indicated surface marker (analysis was performed on CD45+ cells gated by forward and side scatter properties as described in Materials and Methods) and the standard deviation determined from the three samples analyzed for each group. Values that differed significantly between the KIM5 and YopM− groups are in bold. NA, not available.