Fig. 6.

a Changes in tumor volumes of B16 tumor-bearing mice after first treatment. b Tumor volumes in each group at 20th day post administration. Tumor volume was estimated as half of the small diameter squared times the large diameter. c Changes in body weights of B16 tumor-bearing mice after first treatment. d The Ki-67 immunohistochemical stain of tumor tissue at 20th day after first treatment, representing the changes of cell proliferation in tumor tissue. Au(G) AuNPs loaded hydrogel, IR radiation, IR + Au(G) AuNPs loaded hydrogel with radiation, IR + DOX(G) DOX loaded hydrogel with radiation, IR + Au + DOX(G) AuNPs and DOX co-loaded hydrogel with radiation, IR + Au(S) AuNPs solution with radiation, IR + DOX(S) DOX solution with radiation, IR + Au + DOX(S) AuNPs solution and DOX solution mixture with radiation. ^# p < 0.05, ^## p < 0.01, statistically significant compared with saline treatment