FIG. 2.

Effect of ovariectomy (OVX) and estrogen replacement (OVX-E₂) on Agtr1 and Agtr2 protein and mRNA abundance in normoxic females. LVs were isolated from 4-mo-old normoxic females. A) Agtr1 and Agtr2 protein abundance was determined by Western blot analysis (n = 4). B) Agtr1a, Agtr1b, and Agtr2 mRNA abundance was determined by real-time RT-PCR (n = 5). Data are means ± SEM. Data were analyzed by one-way ANOVA followed by Neuman-Keuls posttests, within each receptor subtype. *P < 0.05, OVX versus sham control or E₂ replacement.