FIG. 3.
Effect of ovariectomy (OVX) and estrogen replacement (OVX-E2) on Prkce and Prkcd protein and mRNA abundance in normoxic females. LVs were isolated from 4-mo-old normoxic females. A) Protein abundance of Prkce and Prkcd, was determined by Western blot analysis (n = 4). B) Prkce and Prkcd mRNA abundance was determined by real-time RT-PCR (n = 5). Data are means ± SEM. Data were analyzed by one-way ANOVA followed by Neuman-Keuls posttests, within each isozyme. *P < 0.05, OVX versus sham control or E2 replacement.