FIG. 2.

Rhox8 encodes a Sertoli-specific homeobox factor. Serial testis sections from PND 12 mice were incubated with either preimmune serum (1:250 dilution; A) or RHOX8 polyclonal antiserum (1:2500 dilution; B) from IMGENEX rabbit #2223B immunized with a RHOX8 amino domain peptide. DAB visualization reveals that RHOX8 protein is localized to Sertoli cells as compared to the established Sertoli cell markers RHOX5 and SOX9 (C and D). In adult testes (E–G), RHOX8 staining is present in the nuclei of all Sertoli cells (arrowheads). H–J) Immunofluorescence analysis confirms colocalization of RHOX8 and SOX9 in all Sertoli cells at PND 12. * indicates that nonspecific autofluorescence in erythrocytes was observed in the green channel.