FIG. 5.

RHOX8 is expressed in caput and caudal regions of the adult epididymis. A) Rhox8 is highly expressed in the caput and caudal epididymis as assessed by qPCR. B–F) Epididymal sections from adult mice were incubated with either preimmune serum (1:250 dilution; B) or RHOX8 polyclonal antiserum (1:600 dilution; C and D). RHOX8 protein is localized to the basally located nuclei (arrowheads) of luminal epithelium of the caput (C and D) and caudal (E and F) epididymis. Taken together, these results indicate that Rhox8 expression is not disturbed in RHOX8-KD mice.