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. 2016 Jan 8;11(1):e0145806. doi: 10.1371/journal.pone.0145806

Fig 5. Analysis of phospholipids extracted from strains transformed with mutated versions of plant CGI-58.

Fig 5

A) TLC analysis of total lipids extracted from strains MN7 and BL21(DE3). Lipids were extracted and separated as described in Fig 4. Experiments were carried out at the same time and under the same conditions and, for comparison, TLC pictures of wild-type AtCGI-58 Trc were duplicated from Fig 4. Each experiment was repeated at least twice. B) Comparison of the PE content of SM2-1 transformants determined by LC/MS/MS. Total lipids were extracted and analyzed. The quantities of PE were calculated based on a 36:2 PE standard curve and normalized to the PE content of the wild-type (AtCGI-58 Trc). Values ± SD are the mean of three independent clones. C) Comparison of the PG content of SM2-1 transformants determined by LC/MS/MS. Total lipids were extracted and analyzed. The quantities of PG were calculated based on a 28:0 PG standard curve and normalized to the PG content of the wild-type (AtCGI-58 Trc). Values ± SD are the mean of three independent clones. ** P<0.01, (vs wt).