Table 1. List of primers used.

Restriction sites are underlined, start codons are in bold, and mutated bases for site-directed mutagenesis are italicized.

Primer name	Target	Sequence 5→3’
01	MmCGI-58 Fwd	CAGTCAACATGTTAGCGATGGCGGCG
02	MmCGI-58 Rev	TGCTGCGGCCGCTCAGTCTACTGTGTGGC
03	AtCGI-58 FL Fwd	GTCACATATGAACTTGAGCCGTTTTGCTT
04	AtCGI-58 FL and Trc Rev	TGCTGCGGCCGCCTAAACCAATCGTAGACC
05	AtCGI-58 Trc Fwd	GTCACATATGAAATCAAGATGGAAAATTTTGTG
06	TetC Fwd	ATGAAATCTAACAATGCGCTCATC
07	T7RNA polymerase Rev	GTGCAATCATCTTAGGGAGTA
08	plsC Fwd	TCACCATGGTATATATCTTTCGTCTTATTATTACCG
09	plsC Rev	TGCTGCGGCCGCTTAAACTTTTCCGGCGGCTTC
10	pgsA Fwd	TCAACATGTCATTTAATATCCCTACGTTGCTTAC
11	pgsA Rev	TGCTGCGGCCGCTCACTGATCAAGCAAATCTGCA
12	AtCGI-58 Trc S199N Fwd	CATAATTTTGGAGGCTATGTTGCTAAA
13	AtCGI-58 Trc S199N Rev	ATAGCCTCCAAAATTATGTCCTAATAG
14	AtCGI-58 Trc H379A Fwd	CCACAGGGTGGTGCTTTTGTGTTCATA
15	AtCGI-58 Trc H379A Rev	TATGAACACAAAAGCACCACCCTGTGG
16	AtCGI-58 Trc D384A Fwd	TTTGTGTTCATAGCCAACCCAATTGGT
17	AtCGI-58 Trc D384A Rev	ACCAATTGGGTTGGCTATGAACACAAA
18	CotA Fwd	CAGTCAACATGTCACTTGAAAAATTTGTGGATGCT
19	CotA Rev	GATGCTGCGGCCGCTTATTTATGGGGATCAGTTATATCC