FIG. 1.

Effect of CpG ODN 1826 pretreatment on bacterial load expressed as CFU (A), IFN-γ levels (B), and TNF-α levels (C). BALB/c mice were injected with either CpG ODN 1826 (○) or PBS (•) on day −2 relative to the time of B. pseudomallei challenge (50 LD₅₀) on day 0. Blood was collected on day 2, and each specimen was used for colony count and cytokine assays. Results from individual mice (n = 15 animals per group) and group means (horizontal line) are shown. Data are representative of three independent experiments. Differences between the CpG and control groups were statistically different for panels A (P = 0.0001) and B (P = 0.0002) by the Mann-Whitney U test.