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. 2016 Jan 8;11(1):e0146138. doi: 10.1371/journal.pone.0146138

Fig 5. Effects of vimentin on the MBP-induced progesterone secretion.

Fig 5

(A and B), MLTC-1 and Y1 cells were transfected by con-siRNA or vimentin-siRNA for 12 h, respectively, qRT-PCR (top) and Western blots (bottom) analysis of the expressions of vimentin mRNA and protein. (C and D), after MLTC-1 and Y1 cells were transfected by con-siRNA or vimentin-siRNA for 12 h, they were exposed to100 U/L hCG or 10 μM forskolin in the absence or presence of 1000 nM MBP for 24 h, respectively. The secretion of progesterone was determined in triplicate. The amounts of progesterone measured in control cells were MLTC-1 (hCG): 8.32±0.22 ng/ml, MLTC-1 (hCG+MBP): 10.10±0.42 ng/ml, Y1 (For): 8.64±0.33 ng/ml, and Y1 (For+MBP): 10.31±0.21 ng/ml respectively. **p<0.01 compared with cells transfected by con-siRNA.