Table 1. Primers used in PCR and quantitative RT- PCR.
| Gene | Primer sequence | Length (bp) | GenBank accession no. |
|---|---|---|---|
| cagAa | Sense: 5′-ACAATGACTAACGAAACCA-3′ | 3467 | GQ161098 |
| Antisense: 5′-TTTTGGTAT TCCTTAATCCT-3′ | |||
| cagAb | Sense: 5′-AATACACCAACGCCTCCAAG-3′ | 397 | GQ161098 |
| Antisense: 5′- AAAAGCTTCTGCAGAGCTGGGAGGTGTG-3′ | |||
| β-ACTIN | Sense: 5′-TGGAGAAAATCTGGCACCAC-3′ | 190 | BC 016045 |
| Antisense: 5′-GAGGCGTACAGGGATAGCAC-3′ | |||
| LDH | Sense: 5′-ACGTCAGCAAGAGGGAGAA-3′ | 194 | NM_005566.3 |
| Antisense: 5′-AACCGCTTCCAATAACACG-3′ | |||
| DLD | Sense: 5′-TTCCCATTTGCTGCTAACA-3′ | 214 | NM_000108.3 |
| Antisense: 5′-CTGATAAGGTCGGATGTGC-3′ | |||
| PRPF19 | Sense: 5′-GGACGGAGATTCTTCACTTTACAG-3′ | 183 | NM_014502.4 |
| Antisense: 5′-CAAACCCTAATTCTACCCCTCTACT-3′ | |||
| ATP synthase | Sense: 5′-GCTGCCACTCAACAACTT-3′ | 113 | NM_001001937.1 |
| Antisense: 5′-AGACCCGCATAGATAACAG-3′ | |||
| CaM | Sense: 5′-TGGAGACGGACAAGTCAACTAT-3′ | 243 | NM_006888.3 |
| Antisense: 5′-GACAGGACCACCAACCAATAC-3′ | |||
| p64 CLCP | Sense: 5′-CCTTTGCCACTTGCTCAT-3′ | 198 | NM_001288.4 |
| Antisense: 5′-CCTTTGCCACTTGCTCAT-3′ | |||
| RanGAP | Sense: 5′-AGGAGGAAGATGAGGAAGAGG-3′ | 180 | NM_002883.2 |
| Antisense: 5′-AAGCCAGGAAGGTGGAGAC-3′ | |||
| calreticulin | Sense: 5′-GCCGAGCCTGCCGTCTACTT-3′ | 238 | NM_004343.3 |
| Antisense: 5′-TGAACTGCACCACCAGCGTCT-3′ | |||
| HPRT | Sense: 5′-TGAGGATTTGGAAAGGGTGT-3′ | 118 | NM_000194.2 |
| Antisense: 5′-GAGCACACAGAGGGCTACAA-3′ |
a Primers used to amplify full-length cagA.
b Primers used to detect cagA expression in cells. LDH, L-lactate dehydrogenase. DLD, Dihydrolipoamide dehydrogenase. PRPF19, pre-mRNA processing factor 19 homolog. CaM, calmodulin. p64 CLCP, nuclear chloride ion channel protein. RanGAP, Ran-specific GTPase-activating protein. HPRT, Hypoxanthine-guanine phosphoribosyltransferase.