Table 2. Primers used to amplify promoter CpG islands in methylation-PCR.
Gene | Primer sequence (5′-3′) | Length (bp) |
---|---|---|
Hp16S rRNA | Sense: 5′-GCTAAGAGAT CAGCCTATGTC-3′ | 118 |
Antisense: 5′- CCGTGTCTCAGTTCCAGTGT-3′ | ||
LDH1 | Sense: 5′-CGGAATAAGGATATGATAGGT-3′ | 427 |
Antisense: 5′-ATCCCTAACTATCTCCTAACTTT-3′ | ||
LDH2 | Sense: 5′-GGGGTATTTATTAGGTTTGAAGTT-3′ | 532 |
Antisense: 5′-CCTCCTAAAAA TTCACCCATC-3′ | ||
DLD1 | Sense: 5′-TGTGGATATAGGAGGTGA ATTTT-3′ | 447 |
Antisense: 5′-CAATCAAATCCCAAAAACAATA-3′ | ||
DLD2 | Sense: 5′-TTTATATGGTTGTTGTAAGGATGAA-3′ | 473 |
Antisense: 5′-CCTTAACCAAAAAACAATACACAC-3′ | ||
RanGAP1 | Sense: 5′-TTAGTATAGTGGTATGGATGGTAGG-3′ | 314 |
Antisense: 5′-TCTACTAACCCAACCCTACTCTATT-3′ | ||
RanGAP2 | Sense: 5′-GGGATTGATAGGATATATGGGAT-3′ | 301 |
Antisense: 5′-AATAAATCTAACACCAAAATAACCC-3′ | ||
CaM1 | Sense: 5′-AAGAGGATTAATTTTTTTTAGGAGG-3′ | 250 |
Antisense: 5′-CAACCTCACCCCACCTAAATA-3′ | ||
CaM2 | Sense: 5′-GTTGAGGTGGGAGGGTTATTTA-3′ | 392 |
Antisense: 5′-TCCCAACACCACTACCGAA-3′ | ||
CaM3 | Sense: 5′-TTTTGGTAGTGGTGTTGGGA-3′ | 285 |
Antisense: 5′-AACAAACAAAACAACTAAAAATCTAAA-3′ |
Hp, H. pylori; LDH, L-lactate dehydrogenase; DLD, Dihydrolipoamide dehydrogenase; RanGAP, Ran-specific GTPase-activating protein; CaM, calmodulin.