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. 2016 Jan 8;11(1):e0146521. doi: 10.1371/journal.pone.0146521

Table 2. Primers used to amplify promoter CpG islands in methylation-PCR.

Gene Primer sequence (5′-3′) Length (bp)
Hp16S rRNA Sense: 5′-GCTAAGAGAT CAGCCTATGTC-3′ 118
Antisense: 5′- CCGTGTCTCAGTTCCAGTGT-3′
LDH1 Sense: 5′-CGGAATAAGGATATGATAGGT-3′ 427
Antisense: 5′-ATCCCTAACTATCTCCTAACTTT-3′
LDH2 Sense: 5′-GGGGTATTTATTAGGTTTGAAGTT-3′ 532
Antisense: 5′-CCTCCTAAAAA TTCACCCATC-3′
DLD1 Sense: 5′-TGTGGATATAGGAGGTGA ATTTT-3′ 447
Antisense: 5′-CAATCAAATCCCAAAAACAATA-3′
DLD2 Sense: 5′-TTTATATGGTTGTTGTAAGGATGAA-3′ 473
Antisense: 5′-CCTTAACCAAAAAACAATACACAC-3′
RanGAP1 Sense: 5′-TTAGTATAGTGGTATGGATGGTAGG-3′ 314
Antisense: 5′-TCTACTAACCCAACCCTACTCTATT-3′
RanGAP2 Sense: 5′-GGGATTGATAGGATATATGGGAT-3′ 301
Antisense: 5′-AATAAATCTAACACCAAAATAACCC-3′
CaM1 Sense: 5′-AAGAGGATTAATTTTTTTTAGGAGG-3′ 250
Antisense: 5′-CAACCTCACCCCACCTAAATA-3′
CaM2 Sense: 5′-GTTGAGGTGGGAGGGTTATTTA-3′ 392
Antisense: 5′-TCCCAACACCACTACCGAA-3′
CaM3 Sense: 5′-TTTTGGTAGTGGTGTTGGGA-3′ 285
Antisense: 5′-AACAAACAAAACAACTAAAAATCTAAA-3′

Hp, H. pylori; LDH, L-lactate dehydrogenase; DLD, Dihydrolipoamide dehydrogenase; RanGAP, Ran-specific GTPase-activating protein; CaM, calmodulin.