FIG. 3.

NOS enzyme activity in cultured PBMCs. Venous blood (3 ml) was obtained and PBMCs were collected from healthy Gabonese children (HC; n = 26) and children with malaria (MAL; n = 14). PBMCs (10⁶ cells/ml) were cultured for 7 days with medium alone (control [Con]) or LPS (100 ng/ml) and IFN-γ (200 U/ml). Cell lysates were prepared from cultured PBMCs, and NOS enzyme activity (in picomoles of citrulline per milligram of protein) was determined. The graph shows means ± SEM of results for each of the groups. Statistical significance was determined by the Mann-Whitney U test. *, P value of <0.05 compared to results for HC; **, P value of <0.01 compared to results with unstimulated PBMCs from children with malaria.