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. 2016 Jan 8;11(1):e0146646. doi: 10.1371/journal.pone.0146646

Fig 4. MiR-124 can directly target RHOA mRNA and regulate its expression.

Fig 4

(A) QRT-PCR (up) and western blot (down) analysis of RhoA expression in ADMSCs on day 0, 5 and 10 during the transdifferentiation induction. (B) Predicted binding site between 3’UTR of RhoA mRNA and miR-124 and the designed mutant sequence. (C) Dual luciferase assay of relative luciferase activity of pGL3-RhoA-WT (RhoA-WT) and pGL3-RhoA-MUT (RhoA-WT) transfected with 50 nM miR-124 mimics or the negative control in HEK 293T cells. Firefly luciferase activity was normalized to that of Renilla luciferase. (D) QRT-PCR (up) and western blot (down) analysis of RhoA expression in ADMSCs infected with the pLV-miR-124 locker lentiviral particles or the negative control. * p<0.05, ** p<0.01, *** p<0.001.