Fig 1. Experimental design.

Male wild type (WT) and Tet-mev-1 mice had been continuously supplied with drinking water containing doxycycline (Dox) at a concentration of either 0.1 mg/mL during the prenatal period (received through their mothers) or 0.4 mg/mL after weaning. They were divided into four groups (n = 8 in each group) which were subsequently fed a control, normal diet (ND) or a high-fat/high-sucrose diet (HFHSD) at approximately either 1 (upper) or 2 years of age (lower). The mice were sacrificed under isoflurane anesthesia at the end of the 4-month feeding period, and the obtained serum and liver specimens were subjected to further analyses.