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. Author manuscript; available in PMC: 2016 Jan 8.
Published in final edited form as: Neuroscience. 2010 Jul 16;170(2):441–452. doi: 10.1016/j.neuroscience.2010.07.021

Figure 8. Synapsin promotes a formation of enlarged vesicles and quanta.

Figure 8

A. Endosome-like structures (arrowheads) formed upon sustained depolarization.

B. Tracing several endosome-like structures (arrowheads) through subsequent serial sections demonstrates that they are not connected to the plasma membrane or mitochondria.

B. Potentiation activates formation of endosome-like structures/enlarged vesicles in WT but not in synapsin (−) boutons. Asterisk indicate significant difference (p<0.05) between WT and synapsin (−) genotypes upon potentiation. Dotted lines – before stimulation; bars – after stimulation.

C. High K+ treatment drastically increases the number of endosome-like structures/enlarged vesicles in WT boutons, and this effect is not as prominent in synapsin (−) boutons. EM data collected from 3 preparations (16-19 boutons) for each line at each condition.

D, F. After high K+ treatment, quantal size significantly increases in synapsin (+) but not in synapsin (−) boutons, as could be seen from mean mEPSP amplitudes (D) or those in individual experiments (F). Asterisks indicate significant difference (p<0.05) between WT and synapsin (−) high K+ treated preparations. Data collected from 22 WT and 26 synapsin (−) preparations.