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. Author manuscript; available in PMC: 2016 Feb 1.
Published in final edited form as: J Orthop Res. 2014 Oct 28;33(2):174–184. doi: 10.1002/jor.22749

Figure 2.

Figure 2

SDF-1β mRNA and protein levels in Tet-Off-SDF-1β BMSCs are significantly increased relative to controls, which enhances BMSC osteogenic differentiation in vitro. (A) Normalized SDF-1β mRNA, (B) intracellular SDF-1β protein, and (C) extracellular SDF-1β protein levels. Insets show normalized SDF-1α mRNA and protein levels, respectively controls (24 h, ± 100 ng/ml Dox). (D) Representative Alizarin Red S (ARS)-stained wells (21 days, ± 100 ng/ml Dox). (E) Colorimetric quantification of extracted ARS at 570 nm. Tet-Off-SDF-1β or Tet-Off-EV BMSCs (± Dox) (***p <0.0001 Tet-Off-SDF-1β (−Dox) vs. Tet-Off-SDF-1β (+Dox) or Tet-Off-EV (±Dox), n = 3 per group).