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. Author manuscript; available in PMC: 2017 Feb 1.
Published in final edited form as: Biomaterials. 2015 Dec 2;80:68–79. doi: 10.1016/j.biomaterials.2015.11.051

Table 1. Uptake efficiency of purified GFP fusion proteins in human cell lines.

The relative delivery efficiency of GFP to human cell lines by three different tags was compared by counting the number of cells showing GFP signals under confocal microscope at 100x magnification. A total of 15–20 images were observed for each cell line. All cell lines were examined in triplicate.

CTB-GFP PTD-GFP DCpep-GFP
Human Dendritic cell 70.4 % (19/27) 0% (0/12) 83% (10/12)
Human T cell 91.7 % (11/12) 0% (0/18) 0% (0/13)
Human B cell 87.5 % (14/16) 0% (0/14) 0% (0/10)
Human macrophage cell 91.7 % (11/12) 0 % (0/12) 0 % (0/12)
Human mast cell 91.7 % (11/12) 0 % (0/12) 0% (0/11)
Human Kidney cell 83.3 % (12/13) 83.3 % (10/12) 0% (0/10)
Human pancreatic cells 89.5 % (17/19) 81.2% (9/11) 0% (0/12)