Table II.

Effect on the hematological parameters (systemic toxicity) following 6h or 15 day exposure to milk exosomes in Sprague-Dawley rats.

Hematological profile	6h Exposure		15 day Exposure
	Control	Milk Exo	Control	Milk Exo
WBC	5.7 ± 2.5	6.5 ± 1.6	5.7 ± 1.7	7.0 ± 2.0
HGB	14.0 ± 1.2	13.5 ± 0.9	14.0 ± 0.3	13.8 ± 0.6
HCT	44.0 ± 3.5	41.5 ± 3.4	43.3 ± 2.1	42.3 ± 1.9
MCV	60.8 ± 1.0	59.0 ± 1.8	45.5 ± 29.0	59.3 ± 1.7
MCHC	31.8 ± 0.5	32.4 ± 1.0	32.3 ± 2.3	32.8 ± 0.5
Platelet Count	689.0 ± 145.8	605.8 ± 433.2	755.7 ± 134.9	772.8 ± 85.8
Neutrophils	12.0 ± 3.5	32.7 ± 35.8	11.5 ± 3.0	13.5 ± 8.4
Lymphocytes	84.5 ± 4.5	50.8 ± 40.4	86.0 ± 2.8	81.0 ± 7.4

Female Sprague Dawley rats (6-7) weeks old were provided control diet (AIN 93M) and water ad libitum and treated with milk-derived exosomes (25 mg/kg, b. wt.) by oral gavage for 6 h or 15 days, once daily. At euthanasia blood was collected and analyzed using an automated AU640® Chemistry Analyzer (Beckman Coulter, Inc., Brea, CA, USA) by Antech diagnostics. Data represent average and Sprague-Dawley of four animals. Statistical analysis was performed by student t-test and no significant change was observed in milk exosome treated animals compared to control group.