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γδ T-cell bioactivity of mycoplasma species. Human PBMCs were cultured with serial dilutions of LMW extracts or of synthetic HMB-PP. Outgrowth of Vγ9+ CD3+ cells within the lymphocyte gate was monitored after 6 days by flow cytometry. The plots show representative results obtained for M. genitalium and M. penetrans at dilutions corresponding to the indicated protein concentrations in comparison with those for the medium control and HMB-PP.
