TABLE 1.
Presence of the MEP pathway in selected mycoplasma species
| Mycoplasma species | Genome accession no. | Result by:
|
Culture mediumc | Bioactivity (μg of HMB-PP/g of protein)d | |
|---|---|---|---|---|---|
| Homology searcha | PCR analysisb | ||||
| M. arthritidis | NC_004819 | − | − | BEa | <0.006 |
| M. gallisepticum | NC_004829 | + | + | BEg | 55.1 ± 26.9 |
| M. genitalium | NC_000908 | − | − | SP4 | <0.021 |
| M. penetrans | NC_004432 | + | + | BEg | 5.2 ± 1.6 |
| SP4 | 4.1 ± 0.5 | ||||
| M. pneumoniae | NC_000912 | − | − | SP4 | <0.005 |
| M. pulmonis | NC_002771 | − | − | BEg | <0.012 |
| U. urealyticum | NC_002162 | − | − | SU | <0.015 |
a
Analyzed by TBLASTN, using the sequences of the seven E. coli enzymes depicted in Table 2. Other genomes negative for the MEP pathway include those of M. fermentans, M. mobile, and M. mycoides subsp. mycoides SC (data not shown).
b
Performed on genomic template DNA, using the degenerate gcpE-specific primers ATTATTGCWGATATTCAYTTTAATCCT and GGWCCRTTTACTGMACAACCTAA.
c
Culture media used to obtain cell pellets for subsequent preparation of LMW filtrates (6a).
d
Shown is the mean Vγ9/Vδ2 T-cell bioactivity ± standard error (n = 3) of mycoplasma LMW filtrates as determined with an HMB-PP standard and calibrated against the protein concentration of the original bacterial extract.