Figure 5. Altered mitochondrial functions in the cfo1 mutant.

(A) Susceptibility of the strains to hydrogen peroxide (H2O2) was tested. Ten-fold serial dilutions of cells (starting at 10⁴ 26 cells) were spotted onto plates and incubated at 30°C for 2 days. (B) The growth of the strains in media containing either glucose (YPD, repressing condition) or galactose (YPG, induction condition) as a carbon source and in the presence of fluconazole was monitored. Ten-fold serial dilutions of cells (starting at 10⁴ cells) were spotted onto plates, incubated at 30°C for 2 days and photographed. (C) Activity of aconitase in the strains was compared using zymography. Each lane was loaded with 20μg of total protein from the wild-type strain or the cfo1 mutant using 1 native gel conditions, and aconitase activity was measured (upper panel). The nitrocellulose membrane containing the same protein samples was stained with CPTS and a partial image is shown to indicate that the same amount of each sample was loaded (lower panel). A total of three independent experiments were performed and the results were nearly identical. A representative image is displayed.