Figure 6. Differentially expressed genes involved in cellular respiration and intracellular redox state in the cfo1 mutant.

(A) The values in each column indicate the ratio of expression level as follows: WT+F/WT−F: the wild-type strain with fluconazole versus (vs.) wild-type strain without fluconazole; MT−F/WT−F: the cfo1 mutant without fluconazole vs. the wild-type strain without fluconazole; MT+F/WT+F: the cfo1 mutant with fluconazole versus vs. the wild-type strain with fluconazole; MT+F/MT−F: the cfo1 mutant with fluconazole versus vs. the cfo1 mutant without fluconazole. (B) The cfo1 mutant has a reduced ratio of NAD+ /NADH compared to the wild-type strain. *p = 0.021. (C) Susceptibility of the wild-type strain (WT) to fluconazole in combination with DPI was investigated. The growth of the strains in media containing fluconazole with or without DPIwas monitored. Ten-fold serial dilutions of cells (starting at 10⁴ cells) were spotted onto plates, incubated at 30°C for 2 days and photographed.