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. Author manuscript; available in PMC: 2016 Jan 9.
Published in final edited form as: Lab Chip. 2015 Aug 21;15(16):3380–3390. doi: 10.1039/c5lc00688k

Figure 6.

Figure 6

Characterization of water and urea permeability in murine erythrocytes. A. Water permeability measured as in Fig. 5D in erythrocytes from wild-type mice as a function of osmotic gradient size and direction. B. Permeability measurement of urea, acetamide and methylurea in erythrocytes from wild-type mice and mice lacking urea transporter UT-B. Flow rates were 200, 200, 100, 250, 450 μl/h, respectively, for the urea analog (500 mM in PBS) solution, erythrocyte suspension, PBS (center channel), mineral oil (top) and mineral oil (bottom). (bottom) Stopped-flow light scattering measurement of urea, methylurea and acetamide transport in mouse erythrocytes. Solute gradient was 250 mM.