Interspecies complementation of L. longbeachae mutants. (A and B) Complementation analysis of the icmQLl (A) and ligB (B) mutants with HL-60-derived human macrophages. Symbols for panel A: ⧫, wild-type L. longbeachae ATCC 33462; □, icmQLl mutant (MF395) containing the vector; +, icmQLl gene; ▴, icmQLp gene; ○, icmQLm gene. Symbols for panel B: ⧫, wild-type L. longbeachae ATCC 33462; ▪, L. longbeachae ligB mutant (MF385) containing the vector; ▴, L. longbeachae ligB gene; ▵, L. pneumophila icmR gene; ○, L. micdadei migB gene; •, L. micdadei migB and icmQLm genes. The experiments were performed at least three times, and similar results were obtained. The error bars indicate standard deviations.