TABLE 1.
Plasmids used in this study
| Plasmid name | Descriptiona | Reference or source |
|---|---|---|
| pBluescript SK(+) | oriR (ColE1) MCS oriR (M13) Apr | 27 |
| pGS-lac-02 | pAB-1 with promoterless lacZ | 13 |
| pLAW344 | sacB MCS oriT (RK2) CmrloxP oriR (ColE1) AprloxP | 29 |
| pMMB207αB-Km14 | RSF1010 derivative; IncQ lacIq Cmr PtacoriT MCS containing α complementation with mobA::Km | 24 |
| pT18 | oriR (ColEI) T18 fragment of CyaA (amino acids 225-339) Apr | 16 |
| pT25 | oriR (p15A) T25 fragment of CyaA (amino acids 1-224) Cmr | 16 |
| pGS-Lc-35-14 | icmQLp gene in pMMB207αB-Km14 | 31 |
| pGS-Lc-36-14 | icmR gene in pMMB207αB-Km14 | 24 |
| pGS-Lc-37-14 | L. pneumophila icmTS genes in pMMB207αB-Km14 | 24 |
| pKP-T18R | icmR gene in pT18 | This study |
| pKP-T25R | icmR gene in pT25 | This study |
| pKP-T18Q | icmQLp gene in pT18 | This study |
| pKP-T25Q | icmQLp gene in pT25 | This study |
| pMF-SK-long110 | pBluescript SK(+) containing L. longbeachae icmT to icmQLl | This study |
| pMF-long110 | L. longbeachae icmT to icmQLl in pMMB207αB-Km14 | This study |
| pMF-long111 | ligB gene in pMMB207αB-Km14 | This study |
| pMF-long113 | ligB and icmQLl genes in pMMB207αB-Km14 | This study |
| pMF-long114 | icmQLl gene in pMMB207αB-Km14 | This study |
| pMF-ligB-lacZ | ligB regulatory region in pGS-lac-02 | This study |
| pMF-ligB::Km | pMF-SK-long110 containing the Kmr cassette inside ligB | This study |
| pMF-longQ::Km | pMF-SK-long110 containing the Kmr cassette inside icmQLl | This study |
| pMF-longS::Km | pMF-SK-long110 containing the Kmr cassette inside L. longbeachae icmS | This study |
| pMF-ligB::Km-GR | pLAW344 containing the insert of pMF-ligB::Km | This study |
| pMF-longQ::Km-GR | pLAW344 containing the insert of pMF-longQ::Km | This study |
| pMF-longS::Km-GR | pLAW344 containing the insert of pMF-longS::Km | This study |
| pMF-T18-ligB | ligB gene in pT18 | This study |
| pMF-T25-ligB | ligB gene in pT25 | This study |
| pMF-T18-longQ | icmQLl gene in pT18 | This study |
| pMF-T25-longQ | icmQLl gene in pT25 | This study |
| pMF-mic47 | pBluescript SK(+) containing L. micdadei icmT to icmP | This study |
| pMF-mic10 | L. micdadei icmT to icmQLm in pMMB207αB-Km14 | This study |
| pMF-mic18 | icmQLm gene in pMMB207αB-Km14 | This study |
| pMF-mic19 | migB gene in pMMB207αB-Km14 | This study |
| pMF-mic21 | migB and icmQLm genes in pMMB207αB-Km14 | This study |
| pMF-T18-migA | migA gene in pT18 | This study |
| pMF-T25-migA | migA gene in pT25 | This study |
| pMF-T18-migB | migB gene in pT18 | This study |
| pMF-T25-migB | migB gene in pT25 | This study |
| pMF-T18-micQ | icmQLm gene in pT18 | This study |
| pMF-T25-micQ | icmQLm gene in pT25 | This study |
a
MCS, multiple cloning site.