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Workflow for bioinformatic prediction of putative anticancer, cytotoxic and membrane-penetrating peptides (labeled with numbers 1, 2 and 3) obtained from peptidomic analysis of A. fulica mucous fractions. Details were described in the text. CancerPPD (yellow box) was a database of anticancer peptides. ACPP, AntiCP, ToxinPred and CellPPD were the bioinformatics prediction programs (blue boxes).
