Fig. 6.

Structural predictions of the putative anticancer peptides obtained from the F2 and F5 fractions of the A. fulica mucus. These two fractions were separated into four sub-fractions: < 3 kDa, 3–10 kDa, 10–50 kDa and > 50 kDa. The peptide contents of these sub-fractions were analyzed by protein assay and mass spectrometry. All sub-fractions were trypsinized except the < 3 kDa sub-fraction. Putative anticancer peptides and their structures were predicted by bioinformatic programs and shown in this figure. The structures of these peptides were classified into five categories: random coiled, a single helix, helix-consisted loop, β-sheet-consisted loop and short peptide. Putative peptides predicted from the F2 fraction were displayed on the top half, while those of the F5 fraction were shown on the bottom half. The asterisks indicated the cell-penetrating peptides.