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. 2015 Dec 12;6(1):12–22. doi: 10.1016/j.ijpddr.2015.12.001

Fig. 3.

Fig. 3

NOS2 and Pro-inflammatory cytokines are inhibited by benznidazole in hearts of T. cruzi infected mice. IL-6 and IL-1β mRNA levels were analysed by RT-qPCR in total RNA heart extracts of infected mice (15 d.p.i) or infected and Bzl-treated mice (25 mg/kg/day) at 55 d.p.i (A). NOS2 mRNA levels and NOS2 expression were determined in the same conditions as in (A), by RT-qPCR and Western blot with specific primers and antibodies, respectively (B). The RT-qPCR results were normalized against 18S rRNA. Protein levels were normalized against α-actin. Results are expressed as mean of 3 independent experiments (7 mice/group) ± SEM. *P < 0.05, ***P < 0.0001 vs T. cruzi-infected mice; #P < 0.05 vs. control mice. ###P < 0.0001 vs uninfected control mice.