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. 2016 Jan 8;9:4. doi: 10.1186/s13041-016-0185-7

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© Wu et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — A method to determine relative molar ratios of two proteins. To facilitate quantitative measurement of relative molar ratio of two proteins, A and B, an A-B dimer construct is generated and expressed in cells. The lysate from these cells and samples of interest are loaded onto gels, and blotted for A and B separately. Since the A-B dimer contains A and B at a 1:1 molar ratio, the ratio obtained from the dimers on two blots, dA / dB, serves as a calibrator for the ratio obtained from the sample, sA / sB