Table 7.
Nitric oxide content and antioxidant status in high fat diet-fed quails treated with AEE, ACE or AFE for ten weeks (Part I)
| Treatment (/kg/kday) | NO(μmol/ml) | MDA(nmol/ml) | SOD(U/ml) |
|---|---|---|---|
| Control | 34.89 ± 7.09 | 9.33 ± 1.43 | 210.7 ± 25.4 |
| Model | 28.54 ± 6.64* | 12.13 ± 2.22* | 187.1 ± 23.4* |
| Simvastatin 15 mg | 35.79 ± 5.97# | 7.87 ± 1.54# | 235.5 ± 16.8# |
| AEE 1 g | 34.88 ± 7.12# | 9.40 ± 1.31# | 215.5 ± 17.7* |
| AEE 2 g | 35.38 ± 5.60# | 9.30 ± 1.40# | 217.2 ± 18.9* |
| ACE 100 mg | 35.12 ± 5.60# | 9.37 ± 1.19# | 215.3 ± 20.9* |
| ACE 200 mg | 35.84 ± 6.70# | 9.27 ± 1.18# | 216.3 ± 20.8* |
| AFE 150 mg | 35.58 ± 7.91# | 9.27 ± 1.20# | 215.8 ± 17.8* |
| AFE 300 mg | 36.51 ± 7.65# | 9.11 ± 1.55# | 220.2 ± 22.3* |
*statistically different from control group (P < 0.05)
#statistically different from model group (P < 0.05)
Male quails (three weeks old at the beginning of experiments, four weeks old at the beginning of treatments) were subjected to high fat diet (1 % cholesterol and 14 % pork oil) for a total of ten weeks. After ten weeks, quails were sacrificed and blood were collected. Nitric oxide (NO), malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), glutathione (GSH), nicotinamide adenine dinucleotide phosphate (NADPH) and glutathione peroxidase (GSH-Px) were measured with commercial kits following manufacturer’s protocols. Data are expressed as mean ± standard derivation, N = 8