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Visualization of calcein uptake and release. Calcein was co-incubated with CPP-AuNPs and cells for 4 h. The cell within the dotted box was irradiated. Brightfield images show no change of cell morphology after irradiation (d) compared to before (a). b Fluorescent image of cell before irradiation exhibits clear fluorescent spots. c After irradiation some of these spots are still visible, but calcein was released and spread all over the cytoplasm. Scale bars: 20 µm
